Product Name
Taq DNA Polymerase, 5 U / µL (2500 U)
Product Description
CD Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostics PCR applications. The exceptional quality and purity of the enzyme ensures highest performance that is utilized by the diagnostics industry and research labs. The polymerase is suitable for standard and fast PCR applications giving high product yields from various templates with targets of up to 3 kb in size.
Taq DNA polymerase is a thermostable, highly processive 5'→3' DNA polymerase that has low 5'→ 3' exonuclease activity and lacks 3'→5' exonuclease (proofreading) activity. The latter allows incorporation of modified nucleotides.
The enzyme also exhibits deoxynucleotidyl transferase activity that results in the addition of extra A overhang at the 3' ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
Components
5 × 100 µL Taq DNA Polymerase
10 × 1.8 mL 5 × PCR Reaction Buffer
5 ×1.5 mL 50 mM MgCl2
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 72°C in the presence of the reaction buffer.
Advantage / Highlights
• High product yields and robustness in a wide application range
• Highest quality utilized in molecular diagnostics and research
• Exceptionally pure Taq DNA Polymerase for routine and demanding PCR applications
Applications
• Routine and applied PCR up to 3 kb
• RT-PCR
• TA cloning
Storage & Stability
−20°C (until expiry date - see product label)
