Product Name
Cold Sensitive Mutant (CSM) Taq DNA Pol
Product Description
Cold sensitive mutant (CSM) Taq polymerases is a kind of HotStart Polymerase, from amutant E coli.. CSM Taq polymerase, unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq, retains the hot start ability throughout the whole amplification duration, i.e., all cycles. Cold sensitive mutant Taq polymerase is uniquely suited for Hot Start PCR
Advantage / Highlights
•Cold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not active at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq.
•Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.
•Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.
•Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.
Applications
•Hot start PCR amplification
•Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites
•Primer extension of SNP markers
•Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity
•Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield
•Routine diagnostic Hot Start PCR requiring high reproducibility.
•Real-Time PCR
• Multiple PCR
•Generation of PCR products for TA cloning
Storage & Stability
Store at -20°C
