Product Name
HotStarTaq DNA Polymerase (5000 U)
Product Description
HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. HotStarTaq DNA Polymerase is supplied with the unique CD PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided.
Components
1 × 5000 units HotStarTaq DNA Polymerase, 1 × HotStarTaq Buffer Set (1 × 22 mL PCR Buffer, 1 × 40 mL Q-Solution, 1 × 22 mL MgCl2)
Advantage / Highlights
For highly specific amplification with minimal optimization
Minimal optimization requirements
High PCR specificity
Easy handling and room-temperature setup
Applications
HotStarTaq DNA Polymerase is suitable for a wide variety of applications, including challenging applications, such as amplification of:
Complex genomic templates
Complex cDNA templates (e.g., RT-PCR)
Very low-copy targets (e.g., single-cell PCR)
Reactions with multiple primer pairs
