Product Name
HotStarTaq Plus DNA Polymerase
Print (1000 U)
Product Description
The polymerase combines the high specificity, sensitivity, and minimal optimization of HotStarTaq DNA Polymerase, with a fast 5-minute activation time. PCR can be set up at room temperature and reactions can be directly loaded onto a gel, due to novel CoralLoad PCR Buffer, which contains two gel-tracking dyes. The standard CD PCR Buffer is also included for greater convenience. In addition, Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided. The unique kit components and optimized protocols streamLine the PCR procedure.
Components
1000 units HotStarTaq Plus DNA Polymerase, 10 × PCR Buffer, 10 × CoralLoad PCR Buffer, 5 × Q-Solution, 25 mM MgCl2
Advantage / Highlights
For fast and highly specific amplification in all applications:
High PCR specificity with minimal optimization
Fast 5-minute enzyme activation time
Ready-to-load PCR buffer for faster and easier handling
Applications
HotStarTaq Plus DNA Polymerase is highly suitable for a wide variety of applications, including challenging applications such as amplification of:
Complex genomic templates
Complex cDNA templates (e.g., RT-PCR)
Very low-copy targets (e.g., single-cell PCR)
Reactions with multiple primer pairs
