Product Name
Plant Direct PCR Kit (200 r × n)
Product Description
Plant Direct PCR Kit is designed to perform PCR directly from plant leaves, seeds, etc., and can be used for high-throughput screening of
non-polysaccharide and polyphenolic plant samples. The directly engineered DNA polymerase, which maintains high resistance to PCR
inhibitors in plants and extremely high amplification performance, is suitable for amplification of DNA fragments length within 5 kb. The
unique Lysis Buffer A in kit can be used to lyse fresh or frozen plant tissue with easy handling, and the lysate yield can be used as template
with no need of purification. The protective agent contained in the lysate allows the crude product to be efficiently amplified after multiple
freeze-thaw cycles. Amplification can be directly performed as primers and templates are added to pre-prepared 3 × Plant Direct Master
Mix, which reduce pipetting operation and improve the throughput of detection and reproducibility of results.
Components
2 × Plant Direct Master Mix: 1.25 × 4 mL, Plant Direct Lysis Buffer A: 20 mL, Plant Direct Lysis Buffer B:20 mL
Advantage / Highlights
•Unique super-tolerant DNA polymerase: Directly expanded DNA polymerase, which is highly resistant to PCR inhibitors in plants, while maintaining extremely high amplification performance.
•Broad sample compatibility: suitable for the expansion of various common plant leaves and crude seeds.
•Extremely high stability: no significant decrease in activity after repeated freeze-thaw cycles 50 times.
•Convenient operation: No complicated DNA extraction steps, using a small amount of leaves or simple lysis, you can perform PCR, saving a lot of time.
Storage & Stability
Store 2 × Plant Direct Master Mix at -20 ℃;
Plant Direct Lysis Buffer can be stored at -20 ℃ or 2-9 ℃
