Product Name
One Step Mouse Genotyping Kit
Product Description
One Step Mouse Genotyping Kit is specially designed for the rapid genotyping of mouse and rat, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. This Kit supplies 2 × Taq Plus Master Mix (Dye Plus) (P212), which includes a high-performance Taq Plus DNA Polymerase, dNTP, and optimized buffer system to ensures high amplification efficiency of target DNA.
Components
1 × Mouse tissue Lysis Buffer: 40 mL, Proteinase K: 800 μL, 2 × Taq Plus Master Mix (Dye Plus): 5 mL, 25 mM MgCl2: 500 μL, 5 × PCR Enhancer: 2 mL
Advantage / Highlights
•Specialized lysate based on proteinase K.
•The lysate is used directly for PCR amplification without the need to extract the genome.
•Widely used for amplification of target fragments within 2 kb, and for multiplex PCR reactions with up to 4 primer pairs.
Applications
•Direct PCR reaction from animal tissue
Storage & Stability
Store 1 × Mouse tissue Lysis Buffer at 4℃; store the remaining components at -20℃.
