Product Name
One Step U + Probe Mouse Genotyping Kit
Product Description
One Step U+ Probe Mouse Genotyping Kit is designed for rapid genotyping of mouse genotypes. The kit comprises a whole set of animal
tissue DNA extraction and anti-contamination probe method QPCR amplification system, which is can be used to rapidly release genomic
DNA from tissues such as mouse tail, ear and toe. The product is directly subjected to QPCR amplification, avoiding multiple operations
such as capping and pipetting. The genotype results are directly analyzed by software to avoid manual checkup that can greatly reduce
the time spent on the experiment. The dUTP / UDG anti-contamination system in the reagents rapidly degrades U-containing contaminants
at room temperature, significantly reducing aerosol contamination and improving detection throughput and reproducibility of results.
Components
1 × Mouse tissue Lysis Buffer: 2 × 20 mL, Proteinase K: 800 μL, 2 × CD U+ Probe Master Mix: 2 × 1.25 mL, 50 × ROX Reference Dye 1: 100 μL, 50 × ROX Reference Dye 2: 100 μL
Advantage / Highlights
•Fast processing of animal tissues.
•The lysate after the brief heating is directly used as a template for quantitativePCR detection based on the heat-sensitive UDG anti-pollution dual-probe method. The corresponding genotype can be directly output by the instrument software to avoid manual verification of data introduction errors.
•Simple and convenient operation, it can effectively prevent pipetting pollution and DNA aerosol pollution.
Applications
•Rapid genotyping based on UDG anti-pollution probe qPCR
Storage & Stability
1 × Mouse tissue Lysis Buffer stored at 4℃;
The remaining components are stored at -20 ℃.
