Product Name
CD PCR Cloningplus Kit (10)
Product Description
The CD PCR Cloningplus Kit provides ready-to-use ligation reactions, which contain linearized cloning vectors that carry U overhangs at each 3' end, allowing PCR products containing 3' end A overhangs to be directly ligated and cloned with high efficiency and speed. The CD PCR Cloningplus Kit also provides competent E coli. cells and SOC medium for efficient transformation.
Components
For 10 reactions: 2 × Ligation Master Mix (50 µL), pDrive Cloning Vector (0.5 µg), Distilled water (1.7 mL), CD EZ Competent Cells (10 tubes, 50 µL each), SOC medium (2 × 1.9 mL)
Advantage / Highlights
For direct cloning of PCR products
Just 40 minutes from PCR product to plated cells
Ready-to-use Ligation Master Mix
High-specificity UA hybridization for efficient cloning
Competent cells supplied with the kit
Immediate plating of transformed competent cells
Applications
The CD PCR Cloningplus Kit is suitable for cloning of any PCR product that has a single A overhang at each 3' end. PCR products generated using Taq and other nonproofreading DNA polymerases can be directly cloned without any preparation. Kits offering proofreading DNA polymerases, such as the HotStar HiFidelity Polymerase Kit and the CD LongRange PCR Kit, generate PCR products with A overhangs, and are also highly suited for direct use with the CD PCR Cloningplus Kit.
