Product Name
Pfu DNA Polymerase
Product Description
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts.
Components
Pfu DNA polymerase (2.5 U / ul), 10 × Pfu buffer, 6 × gel loading buffer.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Advantage / Highlights
•3'-5' exonuclease activity provides a low error rate
•One of the most thermostable DNA polymerases known
•Lack of extendase activity means no unwanted 3’ overhangs
•Optimal for blunt-ended PCR cloning
•Optimum temperature near 75°C
•95% active after 1hour incubation at 98 °C
Applications
•High-fidelity PCR and primer-extension reactions
•PCR cloning and blunt-ended amplification product generation
Storage & Stability
20mM Tris-HCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT，0.1% NP-40 ,0.1% Tween20, 0.2mg / mL BSA, 50% (v / v) glycerol
Store all components at -20°C
