Product Name
CD Max Super-Fidelity DNA Polymerase (500 U)
Product Description
CD Max adds unique elongation factors, specificity promoting factors, and plateau phase depressing factors, which greatly improve the long-range amplification ability, amplification specificity and amplification yield. Two monoclonal antibodies that inhibit 5′ → 3′ polymerase activity and 3′ → 5′ exonuclease activity at room temperature are added to CD Max, enabling highly specific hot-start PCR. The enzyme has 5′ → 3′ polymerase activity and 3′ → 5′ exonuclease activity, and the amplification product is blunt ended.
Components
CD Max Super-Fidelity DNA Polymerase 5 ×100 μL
2 × CD Max Buffer 10× 1.25 mL
dNTP Mix (10 mM each) 5 ×100 μL
10 × Loading buffer 5 ×1.25 mL
Advantage / Highlights
•Super fidelity: its fidelity is 53 times that of Taq DNA Polymerase, 6 times that of Pfu DNA Polymerase, and every 300,000 bases are amplified with less than 5 mismatch
•Faster amplification: Extreme speed of 0.5 sec / kb, 30 sec / kb can efficiently amplify most fragments
•Longer amplification: Simple templates such as plasmids and λ DNA can be effectively amplified up to 40 kb, cDNA can be effectively amplified up to 10 kb, and genome can be effectively amplified up to 20 kb
•Wide adaptability: suitable for a variety of GC content fragment amplification, super tolerance to PCR inhibitors, can be used for direct PCR of a variety of samples
Applications
Using simple templates such as λDNA and plasmids, CD Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, CD Max can effectively amplify fragments up to 20 kb; using cDNA templates, CD Max Can effectively amplify fragments up to 10 kb. The amplification mismatch rate is 1 / 53 of ordinary Taq polymerase and 1 / 6 of Pfu polymerase. In addition, CD Max is resistant to PCR inhibitors and can be used for direct PCR of bacterial, fungal, plant tissue, animal tissue or whole blood samples.
Storage & Stability
Store at -20 °C
