Product Name
CD EVO Super-Fidelity DNA Polymerase (100 U)
Product Description
CD EVO Super-Fidelity DNA Polymerase is an upgraded version of CD Super-Fidelity DNA Polymerase. Compared with the previous generation, CD EVO has added a unique elongation factor and deeply optimized the reaction system to further enhance its amplification stability, fidelity, and ability to expand long fragments. Using simple templates such as λDNA and plasmids, CD EVO can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, CD EVO can also effectively amplify fragments up to 20 kb. The amplification mismatch rate is 1 / 69th of ordinary Taq polymerase, and the amplification speed can reach 15 sec / kb. This enzyme has 5'→ 3' polymerase activity and 3'→ 5' exonuclease activity, and the amplification product is blunt-ended.
Components
CD EVO Super-Fidelity DNA Polymerase (1 U / μL) 100 μL
5 × EVO Buffer (with 10 mM MgCl2) 1 × 1.25 mL
25 mM MgCl2 1 mL
dNTP Mix (10 mM each) 100 µL
5 × PCR Enhancer 500 µL
10 × Loading buffer 1.25 mL
Advantage / Highlights
•Effective amplification of 20 kb genomic fragments and 40 kb plasmid fragments
•100 times higher fidelity than Taq DNA Polymerase and 12 times higher than Pfu DNA Polymerase
•Amplification speed is 4-150 times that of conventional polymerase
•The optimized buffer system is widely applicable to various types of templates, so that you can quickly get the ideal amplification results.
Applications
CD EVO is resistant to PCR inhibitors and can be used for direct PCR of bacterial, fungal, plant tissue, animal tissue or whole blood samples.
Storage & Stability
Store at -20 °C
