Product Name

CD HS Super-Fidelity DNA Polymerase (1000 U)

Cat No.

PCR-0140

Price

Inquiry

Product Description

CD HS Super-Fidelity DNA Polymerase is a hot-start version of CD2 × Super-Fidelity DNA Polymerase, which is based on the original addition and can inhibit 5'→ 3' polymerase activity and 3'→ 5' exonuclease activity at room temperature. The two monoclonal antibodies can be used for highly specific Hot Start PCR. 5 × CD SF reaction buffer has good adaptability for simple or complex template, short or long PCR amplification. The buffer already contains 10 mM Mg²⁺ (1 × final concentration of 2 mM). The reaction system can be optimized using DMSO or MgSO₄ provided with the enzyme. The included PCR Enhancer facilitates the amplification of high GC content fragments.

Components

5 × SF Buffer (with 10 mM MgSO₄) 10 × 100 μL 25 mM MgSO₄) 10 × 1 mL dNTP Mix (10 mM each) 10 × 100 µL CD HS Super-Fidelity DNA Polymerase (1 U / μL) 10 × 100 µL 5 × PCR Enhancer 10 × 500 µL DMSO 10 × 100 µL 10 × Loading buffer 10 × 1.25 mL

Advantage / Highlights

•Added monoclonal antibodies for highly specific hot-start PCR
•Fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase
•Amplification speed is 4-150 times that of conventional polymerase
•The optimized buffer system is widely applicable to various templates
•Provides PCR Enhancer to help amplify high GC content templates

Applications

CD HS Super-Fidelity DNA Polymerase can be used for highly specific Hot Start PCR

Storage & Stability

Store at -20 °C