Product Name
CD Super-Fidelity DNA Polymerase (100 U)
Product Description
CD Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase based on Pfu DNA Polymerase. After genetic engineering of Pfu DNA Polymerase, CD Super-Fidelity DNA Polymerase's processivity It has been greatly improved, and even the very complicated templates can accurately and quickly complete the reaction. Its mismatch rate is 1 / 52 of ordinary Taq enzyme and 1 / 6 of Pfu enzyme, and its amplification speed can reach 15 seconds / kb. 5 × CD SF reaction buffer is well-suited for simple or complex template, short or long PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration of 2 mM). The reaction system can be optimized using DMSO or MgSO4 provided with the enzyme. The included PCR Enhancer facilitates the amplification of high GC content fragments.
Components
5 × SF Buffer (with 10 mM MgSO4) 1.25 mL
25 mM MgSO4 1 mL
dNTP Mix (10 mM each) 100 μL
CD Super-Fidelity DNA Polymerase(1U / μL) 100 μL
5 × PCR Enhancer 500 μL
DMSO 100 μL
10 × Loading buffer 1.25 mL
Advantage / Highlights
• Fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase
• Amplification speed is 4-150 times that of conventional polymerase
• Extensive template adaptability
• The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly.
Applications
CD Super-Fidelity DNA Polymerase has extremely high amplification efficiency and extensive template adaptability, and can be used in almost all PCR reactions.
Storage & Stability
Store at -20 °C
