Product Name
Pfu DNA Polymerase, 2.5 U / µL (500 U | 400 r × n)
Product Description
CD Pfu DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. Pfu DNA Polymerase exhibits approximately 10 times higher accuracy than Taq DNA polymerase and amplifies targets up to 3-4 kb in size.
The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction. Additionally the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5'→3' exonuclease activity and no detectable reverse transcriptase activity and produces blunt-end PCR products.
For the most demanding applications, the supplied 5 × PCR Enhancer can be optionally used for improving results when using templates with GC-rich sequences and complex structures.
Components
200 µL Pfu DNA Polymerase
2 × 1.2 mL 10× Pfu Reaction Buffer
1.5 mL 5 × PCR Enhancer
Unit Definition
One unit catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 minutes at 72°C.
Advantage / Highlights
• Accurate PCR for demanding applications
• Approximately ten times higher accuracy than Taq DNA Polymerase
• Proof-reading for increased fidelity
Applications
• High-fidelity PCR
• Generation of PCR products for blunt cloning
• Site directed mutagenesis
Storage & Stability
−20°C (until expiry date - see product label)
