Product Name
Pfu PCR Master Mix, 2 × (500 r × n / 50 µL)
Product Description
CD Pfu PCR Master Mix is a perfect choice for fast, high-fidelity PCR setup that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for routine high-throughput, high-fidelity amplification of targets up to 3-4 kb in size.
The 2 × Pfu PCR Master Mix contains Pfu DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. For the most demanding applications, the supplied 5 × PCR Enhancer can be optionally be used to improve results when using templates with GC-rich sequences and complex structures.
Pfu DNA Polymerase exhibits approximately 10 times higher accuracy compared to Taq DNA polymerase. Pfu DNA Polymerase produces blunt-end PCR products suitable for blunt cloning.
Components
10 × 1.25 mL Pfu PCR Master Mix
1.5 mL 5 × PCR Enhancer
Advantage / Highlights
• Optimized Pfu PCR Master Mix for minimal hands-on and fast setup
• Pure Pfu DNA Polymerase and highest quality dNTPs
• Approximately ten times higher accuracy than Taq DNA Polymerase for accurate PCR in demanding applications
Applications
• High-throughput, high-fidelity PCR
• Generation of PCR products for blunt cloning
Storage & Stability
−20°C (until expiry date - see product label)
