Product Name
Long Range DNA Polymerase, 2.5 U / µL (500 U | 400 r × n)
Product Description
CD Long Range DNA Polymerase is a first-choice for amplification of targets up to 40 kb in size with higher accuracy than Taq DNA polymerase.
This specially designed blend of thermophilic polymerases is well suited for amplification of targets that are GC-rich and have complex structures.
For the most demanding applications, the supplied 5 × PCR Enhancer can be optionally used to improve results when using templates with GC-rich sequences and complex structures.
Long Range DNA Polymerase produces a mixture of A-tailed and blunt-end PCR products. It is advisable to blunt products before cloning into blunt-end vectors.
Components
5 × 40 µL Long Range PCR Enzyme Mix
5 × 400 µL 10× Long Range Reaction Buffer
1.5 mL 5 × PCR Enhancer
Unit Definition
One unit catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 minutes at 72°C.
Advantage / Highlights
• High-productivity, long-range PCR
• Increased fidelity for accurate amplification of GC-rich templates
• Polymerase mix for high yield and short cycle times
Applications
• Long-range PCR up to 40 kb
• Amplification of GC-rich templates
Storage & Stability
−20°C (until expiry date - see product label)
