Product Name
Long Range PCR Master Mix, 2 × (500 r × n / 50 µL)
Product Description
CD Long Range PCR Master Mix is a perfect choice for fast reaction setup for long-range PCR that reduces the time required for calculation and pipetting and eliminates the need for buffer optimization. It is designed for amplification of targets up to 40 kb in size. The master mix works well with GC-rich templates and amplifies DNA with a higher fidelity than Taq DNA polymerase.
The Long Range PCR Master Mix contains a blend of thermophilic polymerases, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added.
For the most demanding applications, the supplied 5 × PCR Enhancer can be optionally used to improve results when using templates with GC-rich sequences and complex structures.
Long Range PCR Master Mix produces a mixture of A-tailed and blunt-end PCR products. It is advisable to blunt products before cloning into the blunt-end vector.
Components
10 × 1.25 mL Long Range PCR Master Mix
1.5 mL 5 × PCR Enhancer
Advantage / Highlights
• Optimized Long Range PCR Master Mix for minimal hands-on and fast setup
• Mix of pure polymerases and highest quality dNTPs for high yield and short cycle times
• Increased fidelity for accurate amplification of GC-rich templates
Applications
• Long-range PCR up to 40 kb
• Amplification of GC-rich templates
Storage & Stability
−20°C (until expiry date - see product label)
