Product Name

UCP HiFidelity PCR KitPrint (500)

Cat No.

PCR-0153

Price

Inquiry

Product Description

The UCP HiFidelity PCR Kit provides a convenient format for highly sensitive and specific hot start PCR that need to have low PCR-error rates using any DNA or cDNA template. Dedicated processes enable ultra clean production of this master mix. These processes deplete potential background DNA to provide the cleanest sample possible. Combined with superior inhibitor resistance and minimized GC-content bias, this kit is an ideal choice for microbiome applications such as 16S or 18S amplification. This is complemented with a 70 times higher fidelity compared to Taq polymerase, thus enabling error-free amplification, as needed during cloning, amplicon-sequencing or analysis of genome-editing experiments. Additional features include visual pipetting controls, a fast cycling protocol, room-temperature stability during and after reaction setup and the ability to amplify long amplicons.

Components

For 500 × 25 µL PCR reactions: 5 × 1.3 mL UCP HiFidelity MasterMix (2 ×); 2 × 200 μL UCP Template Tracer, 25 ×; 1 × 50 μL UCP Master MixTracer, 125 ×; 4 × 1.9 mL UCP PCR Water

Advantage / Highlights

Ultra-Clean Production master mix for high-fidelity hot-start PCR and microbiome applications: Eliminate residual DNA and background noise with ultra clean, DNA-depleted reagents Acquire reliable, robust results without inhibitor effects over wide GC-content range Exceptionally fast PCR with fidelity 70 times higher than Taq Superior specificity and sensitivity: antibody-mediated hot start of polymerase and exonuclease Visual pipetting control and gel tracking dyes monitor successful procedure Automation ready: stable, room-temperature reaction setup

Applications

The UCP HiFidelity PCR Kit is ideally suited for procedures requiring a high purity and accuracy, like microbial testing, microbiome / metagenome analysis and sequencing, quality control, genotyping and gene editing. The kit creates blunt-end products and is perfectly suited for procedures requiring high-fidelity PCR, such as cloning or site directed mutagenesis. The capability of low degree multiplexing and amplification of long targets up to 10 kb also makes it a perfect choice for amplicon sequencing analysis. The high inhibitor robustness even allows direct PCR from blood