Product Name
Long PCR Taq DNA polymerase
Product Description
Long PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.
Long PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, which can be used in TA clone
Components
Long PCR Taq DNA Polymerase, PCR Enhancer, 6 × gel loading buffer, 10X Long PCR Taq Buffer Ⅰ with Mg2+, 10X Long PCR Taq Buffer Ⅱ with Mg2+
Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT，0.1% NP-40, 0.1% Tween20, 0.2mg / mL BSA, 50% (v / v) glycerol
10X Long PCR Taq Buffer Ⅰ with Mg2+
500mM Tris-HCl pH 8.8，160mM (NH4)2SO4 ，25mM MgCl2 ，1% Triton X-100
10X Long PCR Taq Buffer Ⅱ with Mg2+
200mM Tris-HCl PH8.8，100mM KCl，100mM (NH4)2SO4，16mM MgSO4，1% Tritonx-100
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Advantage / Highlights
• High fidelity: three times fidelity of Taq DNA Polymerase.
• Longer fragment: amplify long templates as long as 40kb.
• Amplification of complex template (GC rich or repetitive sequence).
• Generates 3'-dA and blunt end PCR products.
Applications
• PCR amplification of DNA fragments any sizes around 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
Note
10 ×Long PCR BufferⅠis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.
10 ×Long PCR Buffer Ⅱ is an alternatie long PCR buffer. It is for better fidelity but may not be robust for longer templates above 10kb.
