Product Name
Long PCR Taq Master Mix with Optimizer
Product Description
Long PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.
Long PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, which can be used in TA clone
Components
This product is a 2 × Long PCR reagent Mix, plus a separately supplied Long PCR DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations. 5 × 1 mL is supplied.
Advantage / Highlights
• High fidelity: three times fidelity of Taq DNA Polymerase.
• Longer fragment: amplify long templates as long as 40kb.
• Amplification of complex template (GC rich or repetitive sequence).
• Generates 3'-dA and blunt end PCR products.
Applications
• PCR amplification of DNA fragments any sizes around 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
Note
10 × Long PCR Buffer is classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.
10 × Long PCR Buffer Ⅱ is an alternative long PCR buffer. It is for better fidelity but may not be robust for longer templates above 10 kb.
