Product Name

CD-LAmp DNA Polymerase (Mg²⁺ free buffer, with dNTP, 500 U)

Cat No.

PCR-0162

Price

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Product Description

CD-LAmp DNA Polymerase is a mixed enzyme consisting of Taq DNA Polymerase and a protein containing 3′ → 5′ exonuclease activity, and has six times the fidelity of Taq DNA Polymerase. With a specially optimized buffer system, CD-LAmp DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments of up to 21 kb from the genome and has high amplification efficiency for templates from different sources and different lengths. The 3' end of the PCR product has A and can be cloned into a T vector. The included PCR Enhancer facilitates the amplification of high GC content fragments.

Components

10 × CD-LAmp Buffer (Mg²⁺ free) 2 ×1mL 25 mM MgCI2 2 ×1mL CD-LAmp DNA Polymerase (5 U / μL) 100μL dNTP Mix (10 mM each) 400 μL 5 × PCR Enhancer 500 μL 10 × Loading buffer 2 ×1.25 mL

Advantage / Highlights

• Efficient amplification of genomic and cDNA fragments> 20 kb
• Extremely high amplification sensitivity and strong tolerance to low-purity templates
• Broad GC adaptability
• Provides ready-to-use premixes to minimize experimental steps

Storage & Stability

Store at -20 °C