Product Name
FS Fast DNA Polymerase
Product Description
FSTM Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FSTM PCR Buffer, designed for FSTM Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSTM Taq DNA polymerase is as fast as 5s / kb for simple templates, which shortens the amplification time at least by half.
Components
FSTM Taq DNA Polymerase, 10 × FSTM PCR Buffer, 6 × gel loading buffer
6 × gel loading buffer ：20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol
10X FSTM PCR Buffer：200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Advantage / Highlights
•Fast rate of elongation: elongation rate can reach to 3 kb / min, more than twice the rate by regular Taq DNA polymerase;
•Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products.
Applications
•Routine PCR
•PCR labeling
•PCR sequencing
•Generate PCR product for TA cloning
Storage & Stability
Store all components at -20°C
