Product Name
Fast Polymerase Master Mix with Optimizer
Product Description
This product is a 2 × Fast DNA reagent Mix, plus a separately supplied Fast DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations for efficient PCR amplification. This Optimzer format provides flexibility of optimizing Fast DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes. Fast DNA polymerase is > 2 × faster than Taq
Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FSTM PCR Buffer, designed for FSTM Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSTM Taq DNA polymerase is 2 more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half.
Components
The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Advantage / Highlights
•Fast rate of elongation: elongation rate can reach to 3 kb / min, more than twice the rate by regular Taq DNA polymerase;
•Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products.
Applications
•Routine PCR
•PCR labeling
•PCR sequencing
•Generate PCR product for TA cloning
Storage & Stability
Store all components at -20°C
