Product Name
cDNA Synthesis Kit (250 r × n / 20 µL)
Product Description
CD cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. CD RevertUP II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability.
CD RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb.
For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.
Components
2 × 125 µL Reverse Transcriptase
2 × 1 mL 5 × cDNA Synthesis Buffer
2 × 250 µL dNTP Mix, 62.5 µL RNase Inhibitor
2 × 125 µL Hexamer and 62.5 µL Oligo (dT) Primer
10 × 1.5 mL PCR Grade Water
Advantage / Highlights
• Highly efficient synthesis of long cDNAs (≥ 19 kb)
• Excellent yields at temperatures up to 55°C
• High sensitivity reverse transcription from low abundance template
• Superior performance in demanding applications, including templates with a high degree of secondary structure
Applications
• First-strand cDNA synthesis for RT-PCR and qPCR
• Gene expression profiling
• RNA labeling and primer extension
• cDNA library construction
Storage & Stability
−20°C (until expiry date - see product label)
