Product Name

One Step RT PCR Kit (500 r × n / 50 µL)

Cat No.

PCR-0188

Price

Inquiry

Product Description

CD One Step RT-PCR Kit provides an easy and efficient way to perform both reverse transcription of RNA and PCR amplification of cDNA in one step. Only RNA template, primers and PCR-grade water are added. The 20 × RT-RI Blend, which contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensures high yields of cDNA. The 2 × One Step Mix contains unique Hot Start Taq DNA Polymerase, dNTPs, MgCl₂ and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and difficult templates. PCR enhancers included in the mix allow efficient amplification of complex templates including GC or AT-rich sequences.

Components

10 × 1.25 mL One Step Mix 10 × 125 µL RT-RI Blend

Advantage / Highlights

• Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
• Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
• PCR enhancers allowing sensitive low background amplification

Applications

• One-step RT-PCR
• Virus detection
• Amplification of GC-rich and complex templates

Storage & Stability

−20°C (until expiry date - see product label)