Product Name
MMuLV Reverse Transcriptase, 200 U / µL (50000 U | 250 r × n)
Product Description
CD MMuLV Reverse Transcriptase is an exceptionally pure DNA polymerase which uses RNA as a substrate and exhibits no measurable proofreading 3'→5' exonuclease function. This enzyme performs cDNA synthesis by extending a DNA primer annealed to an RNA template; it can also make copies from a single-stranded DNA templates.
The enzyme is purified from a recombinant E coli. strain carrying the MMuLV reverse transcriptase gene.
Components
5 × 50 µL MMuLV Reverse Transcriptase
5 × 100 µL 10× MMuLV RT Buffer
Unit Definition
One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble fraction in 10 minutes at 42°C when poly(A)+ RNA and oligo (dT)20 are used as template-primer.
Advantage / Highlights
• Pure reverse transcriptase for cDNA synthesis
• High yields of first-strand cDNA
• High value for a fair price
Applications
• First-strand cDNA synthesis
• Generation of labeled cDNA
• RNA analysis by primer extension
Storage & Stability
−20°C (until expiry date - see product label)
