Product Name
Sensiscript RT Kit (200)
Product Description
The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50 ng), such as single-cell RT-PCR and analysis of biopsies and LMD samples. Sensiscript Reverse Transcriptase allows highly efficient RT-PCR over a wide dynamic range and extremely sensitive RT-PCR with very small RNA amounts due to its high affinity for RNA. The Sensiscript RT Kit includes Sensiscript Reverse Transcriptase, dNTPs, and an optimized reaction buffer. Simply add primers for fast and easy cDNA synthesis.
Advantage / Highlights
• For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
• High cDNA yields due to high-affinity enzyme
• Sensitive detection of as few as 10 copies of template
• No additional RNase H digestion step required
• Fast and easy procedure with no tedious pipetting steps
Applications
Sensiscript Reverse Transcriptase is suitable for use in the following applications:
Standard RT and RT-PCR
CDtative, real-time RT-PCR
Differential display RT-PCR
Primer extension and RACE analysis
Synthesis of double-stranded cDNA for cloning
