Product Name
CDTect Reverse Transcription Kit (50)
Product Description
The unique CDTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. All components that are required for fast and efficient reverse transcription are provided with the CDTect Reverse Transcription Kit, including Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, and a unique RT Primer Mix. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts.
Components
For 50 × 20 µL reactions: 100 µL 7 × gDNA Wipeout Buffer, 50 µL Quantiscript Reverse Transcriptase, 200 µL 5 × Quantiscript RT Buffer, 50 µL RT Primer Mix, 1.9 mL RNase-Free Water
Advantage / Highlights
• For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
• cDNA synthesis and gDNA removal in only 20 minutes
• High cDNA yields even from low-abundance transcripts
• cDNA synthesis from 5' and 3' regions of transcripts
• No need to design RNA-specific primers or probes
Applications
The CDTect Reverse Transcription Kit allows highly efficient and sensitive real-time RT-PCR for all types of starting material, including laser-microdissected samples and tissue biopsies.
