Product Name
CDNova Reverse Transcription Kit (200)
Product Description
The unique CDNova Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal and an additional internal control. Genomic DNA contamination in RNA samples is effectively eliminated by the gDNA Removal Mix, and the internal control RNA can be used to test successful reverse transcription and amplification. The CDNova Reverse Transcription Kit provides everything you need for fast and efficient reverse transcription. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts.
Components
For 200 × 20 µL reactions: 4 × 100 µL 8 × gDNA Removal Mix, 4 × 50 µL Reverse Transcription Enzyme, 4 × 200 µL Reverse Transcription Mix (containing RT primers), 4 × 100 µL Internal Control RNA, 4 × 1.9 mL RNase-Free Water
Advantage / Highlights
• For fast cDNA synthesis and reproducible real-time two-step RT-PCR
• Eliminate artifacts using the gDNA Removal Mix
• Monitor successful cDNA synthesis using the internal control
• Synthesize cDNA over a wide linear range with high-affinity transcriptase
Applications
The CDNova Reverse Transcription Kit allows highly efficient and sensitive real-time RT-PCR for all types of starting materials, including laser-microdissected samples and tissue biopsies.