Product Name
CDNova Multiplex RT-PCR Kit (500)
Product Description
The CDNova Multiplex RT-PCR Kit enables fast and reliable quantification of up to 5 RNA targets in a single tube by multiplex real-time RT-PCR. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. Our Q-Bond technology and optimized master mix promote ultrafast multiplex real-time RT-PCR within 1 hour. The combination of a unique two-phase hot-start procedure with our multiplex PCR buffer system ensures highly sensitive qRT-PCR on any real-time cycler without the need for optimization, and enables automated reaction setup at room temperature. The kit also provides protocols for extracted RNA and direct amplification from cultured cells, even down to a single cell. The optional CDNova Internal Control RNA can be used to monitor successful reverse transcription and amplification, and the visual pipetting control can be used to monitor correct pipetting.
Components
For 500 × 20 µL reactions: 2 × 1.3 mL 4 × CDNova Multiplex RT-PCR Master Mix, 100 µL CDNova Multiplex RT-Mix, 100 µL CDNova IC RNA, 500 µL CDNova Yellow Template Dilution Buffer, 1 mL QN ROX Reference Dye, 4 × 1.9 mL RNase-Free Water
Advantage / Highlights
• For fast, reliable quantification of up to 5 RNA targets in a single tube by multiplex real-time RT-PCR
• Sensitive detection of up to 5 targets in 1 tube
• Reliable quantification of low- and high-abundance targets
• Direct amplification from a single cell or up to 800 ng template
• Visual pipetting control to minimize human errors
• Two-phase hot-start procedure for room-temperature setup
• Internal control RNA for verification of successful RT-PCR
Applications
The CDNova Multiplex RT-PCR Kit can be used for multiplex gene expression analysis of RNA targets on any real-time cycler. To fully benefit from multiplexing, we recommend using an instrument that provides up to 5-plex capacity, such as the Rotor-Gene Q.
