Product Name
CD qPCR SYBR Green Master Mix (2500 r × n)
Product Description
CDTaq DNA Polymerase is a chemically modified Taq DNA Polymerase. The activity is completely blocked at room temperature. The activity is released only after heating at 95 °C for 5 minutes, which can effectively prevent non-specific amplification and primers during sample preparation and reaction temperature rise. Dimer. The CD Dye Master Mix is a special reagent for qPCR using the SYBR Green I chimeric fluorescence method. It is based on hot-start CDTaq DNA Polymerase, and with the optimal buffer optimized for qPCR, it can effectively inhibit non-specific amplification and is suitable for high-specificity qPCR reactions. Using this product for qPCR reaction, a good standard curve can be obtained in a wide quantitative region, and the target gene can be accurately quantified and detected with good reproducibility and high reliability.
Components
2 × CDqPCR SYBR Green Master Mix: 20 × 1.25 mL
50 × ROX Reference Dye 1: 5 × 200 μL
50 × ROX Reference Dye 2: 5 × 200 μL
2 × CD qPCR SYBR Green Master Mix: Contains dNTP, Mg2+, CDTaq DNA Polymerase, SYBR Green I, etc.
50 × ROX Reference Dye: It is used to correct the error of fluorescence signal between wells.
Advantage / Highlights
• Hot-start enzyme: CDTaq DNA Polymerase based on chemical hot-start to provide perfect amplification specificity
• Optimized reaction system: Patented buffer formula, to minimize non-specific amplification and primer dimers, without the need to repeatedly optimize conditions
• Excellent amplification performance: repeat reliable quantitative results to meet the data requirements of high-level magazines
Applications
• Suitable for highly specific qPCR reactions
Storage & Stability
Store at -20 °C in the dark
