Product Name
CD Cell SYBR Green Kit
Product Description
The CD Cell SYBR Green Kit accelerates and streamLines real-time RT-PCR and is ideal for experiments requiring rapid high-throughput gene expression analysis.The kit provides a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in SYBR Green-based real-time one-step RT-PCR. A variety of other detection formats are also available, including singleplex detection with sequence-specific probes or multiplex detection with sequence-specific probes (with or without ROX passive reference dye).
Components
FastLane Cell One-Step Buffer Set, 2 × CDTect SYBR Green RT-PCR Master Mix, and CDTect RT Mix
Advantage / Highlights
• For multiplex, real-time one-step RT-PCR analysis from cultured cells without RNA purification
• No RNA purification required, significantly saving time
• Just 3 steps from cells to real-time RT-PCR
• High-throughput analysis of 96- and 384-well plates
• Unique gDNA Wipeout Buffer enables detection of RNA only
• Immediate startup using optimized reagents and protocols
Applications
The CD Cell SYBR Green Kit is ideal for experiments that require rapid, high-throughput gene expression analysis, such as:
Validation of siRNA-mediated gene knockdown
Screening of potential drugs
Detection of stimulated gene regulation
