Product Name

CDTect Virus + ROX Vial Kit (1000)

Cat No.

PCR-0316

Price

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Product Description

The CDTect Virus Kit is specially designed for highly sensitive detection of up to 4 viral nucleic acid targets by multiplex, real-time one-step RT-PCR using sequence-specific probes. Multiple viral RNA and / or DNA targets plus internal controls can be detected in a single tube without loss of sensitivity. The concentrated 5 × master mix enables the addition of higher sample input and contains HotStarTaq Plus DNA Polymerase. The CDTect Virus RT Mix contains a unique formulation of Sensiscript Reverse Transcriptase that is optimized for highly sensitive detection of viral RNA. Two kit formats are available: the CDTect Virus Kit for cyclers that require ROX dye for fluorescence normalization and the CDTect Virus +ROX Vial Kit for other cyclers. For convenience, the master mix can be stored at 2-8 °C.

Components

For 1000 × 50 µL reactions: CDTect Virus NR Master Mix (without ROX dye), ROX Dye Solution, CDTect Virus RT Mix, RNase-Free Water, CDTect Nucleic Acid Dilution Buffer

Advantage / Highlights

• For highly sensitive detection of viral RNA and / or DNA
• High sensitivity in single and multiplex assays
• Detection of viral RNA and / or DNA in the same reaction
• Clear detection of weak positive signals
• Fast universal 2-step protocols
• 5 × master mix for higher sensitivity with more sample input

Applications

CDTect Virus Kits provide highly sensitive real-time single or multiplex PCR or one-step RT-PCR using sequence-specific probes for detection of viral DNA and / or RNA plus internal controls. The kits can be used on a wide range of real-time thermal cyclers, including instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, QIAGEN, Roche (except for capillary cyclers), and Agilent.