Product Name

CDFast Multiplex RT-PCR + R Kit (400)

Cat No.

PCR-0320

Price

Inquiry

Product Description

CDFast Multiplex RT-PCR Kits enable fast and reliable quantification of up to 4 RNA targets in a single tube by multiplex, real-time one-step RT-PCR. A special blend of reverse transcriptases delivers fast and efficient cDNA synthesis. Q-bond technology and an optimized master mix promote fast, multiplex real-time RT-PCR, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. Two kit formats are available: the CDFast Multiplex RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDTect Multiplex RT-PCR +R Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.

Components

For 400 × 25 µL reactions: 3 × 1.7 mL 2 × CDFast Multiplex RT-PCR Master Mix (without ROX dye), 100 µL CDFast RT Mix, 210 µL ROX Dye Solution, 2 × 2 mL RNase-Free Water

Advantage / Highlights

• For fast, multiplex, one-step qRT-PCR using sequence-specific probes for gene expression analysis
• Sensitive detection of multiple RNA targets in 1 well
• Faster results with time savings of up to 50%
• Successful multiplex RT-PCR without the need for optimization
• Precise discrimination of small differences in target amount
• Detection of reference gene and up to 3 targets in the same tube

Applications

CDFast Multiplex RT-PCR Kits can be used for multiplex gene expression analysis of RNA targets on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Multiplex RT-PCR Kit, which has been specially developed for fast cycling on these instruments.