Product Name
CDTect Multiplex RT-PCR NR Kit (200)
Product Description
CDTect Multiplex RT-PCR Kits enable reliable quantification of up to 5 RNA targets in a single tube by multiplex, real-time one-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive and reliable multiplex qRT-PCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. Two kit formats are available: the CDTect Multiplex RT-PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDTect Multiplex RT-PCR NoROX Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.
Components
For 200 × 50 µL reactions: 3 × 1.7 mL 2 × CDTect Multiplex RT-PCR NoROX Master Mix (without ROX dye), 100 µL CDTect Multiplex RT Mix, 2 × 2 mL RNase-Free Water
Advantage / Highlights
• For multiplex, one-step qRT-PCR using sequence-specific probes for gene expression analysis
• Multiplex analysis with no need for optimization
• Analysis of multiple targets in a single reaction
• Sensitive detection of as few as 10 copies of each target
• Reliable quantification of target and reference genes
• Detection of reference gene and up to 3 targets in the same tube
Applications
CDTect Multiplex RT-PCR Kits can be used for gene expression analysis of RNA targets on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Multiplex RT-PCR Kit, which has been specially developed for fast cycling on these instruments.
