Product Name
CDFast Probe RT-PCR Plus Kit (400)
Product Description
The CDFast Probe RT-PCR Plus Kit is highly suited for gene expression analysis of RNA from all sources, including short, fragmented RNA (e.g., RNA from formalin-fixed, paraffin-embedded [FFPE] samples). This kit provides rapid, real-time one-step RT-PCR quantification of 1 or 2 RNA targets in a single tube using probe detection (e.g., using hydrolysis [TaqMan] probes like the type provided with CDFast Probe Assays). In order to avoid false-positive signals, genomic DNA contamination is effectively eliminated through an optimized incubation step prior to real-time RT-PCR. ROX dye is provided in separate tubes at two different concentrations for fluorescence normalization, enabling use of the kit on any real-time cycler. For convenience, the master mix can be stored at 2-8 °C.
Components
For 400 × 25 µL reactions: 1.3 mL 2 × CDFast Mix 1 (x 2), 1.3 mL 2 × CDFast Mix 2 (x 2), 100 µL RT Mix, 210 µL ROX Dye Solution, 210 µL High-ROX Dye Solution, 1.9 mL RNase-Free Water
Advantage / Highlights
• For fast, one-step qRT-PCR using sequence-specific probes, including genomic DNA removal
• Efficient removal of gDNA for accurate gene expression analysis
• Highly suited for analysis of short, fragmented RNA from FFPE samples
• Sensitive detection of two targets simultaneously
• Ready-to-use, real-time one-step PCR master mix
• Fast universal protocol for all cyclers
Applications
The CDFast Probe RT-PCR Plus Kit is highly suited for gene expression analysis of analytes from all sources, including FFPE tissue samples and degraded RNA samples. The kit can be used on fast cyclers with rapid ramping rates as well as on standard cyclers, including instruments from QIAGEN, Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent.
