Product Name
CDNova Probe RT-PCR Kit (2500)
Product Description
The CDNova Probe RT-PCR Kit enables sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. The combination of a unique two-phase hot-start and PCR buffer system in the ready-to-use master mix allows room-temperature setup and ensures highly sensitive qRT-PCR on any real-time cycler. The CDNova Internal Control RNA can be used to test successful reverse transcription and amplification. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. What's more, the visual indicator reduces pipetting error while the integrated gDNA reduction step prevents overquantification of transcripts caused by genomic DNA carryover.
Components
For 2500 × 20 µL reactions: 15 × 1.7 mL CDNova Probe RT-PCR Master Mix, 5 × 100 µL CDNova Probe RT Mix, 5 × 100 µL Internal Control RNA , 5 × 500 µL Yellow Template Dilution buffer, 5 × 1 mL ROX Reference Dye, 10 × 1.9 µL RNase-Free Water
Advantage / Highlights
• For one-step qRT-PCR using sequence-specific probes for gene expression analysis
• Unique two-phase hot-start procedure for room-temperature setup
• Visual pipetting control, resulting in fewer pipetting errors
• gDNA reduction for improved quantification accuracy
• Internal control for positive in-process verification of successful RT-PCR
• Duplex capability, enabling inclusion of internal control or reference gene
• Accurate quantification over several logs of template
Applications
The CDNova Probe RT-PCR Kit can be used for gene expression analysis of RNA targets on any real-time cycler. This includes the CD-Gene Q and instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.
