Product Name
CDTect Probe PCR Kits (1000)
Product Description
CDTect Probe PCR Kits enable sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mix can be stored at 2-8 °C.
Components
For 1000 × 50 µL reactions: 25 mL 2 × CDTect Probe PCR Master Mix, 20 mL RNase-Free Water
Advantage / Highlights
• For real-time PCR and two-step RT-PCR using sequence-specific probes
• Highly sensitive detection of low-copy targets
• Accurate quantification over several logs of template
• Use of any sequence-specific probe on any real-time cycler
• Available with or without uracil-N-glycosylase (UNG)
• No need to optimize reaction and cycling conditions
Applications
CDTect Probe PCR Kits can be used for probe-based gene expression analysis of cDNA targets or quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Probe PCR Kit, which has been specially developed for fast cycling on these instruments.
