Product Name
CDTect Multiplex PCR NoROX Kit (200)
Product Description
CDTect Multiplex PCR Kits enable reliable quantification of up to 5 gDNA or cDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive and reliable multiplex qPCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. Two kit formats are available: the CDTect Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDTect Multiplex PCR NoROX Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.
Components
For 200 × 50 µL reactions: 3 × 1.7 mL 2 × CDTect Multiplex PCR NoROX Master Mix (without ROX dye), 2 × 2 mL RNase-Free Water
Advantage / Highlights
• For multiplex, real-time PCR and RT-PCR using sequence-specific probes
• Multiplex analysis with no need for optimization
• Sensitive detection of as few as 10 copies of each target
• Reliable quantification of target and reference genes
• Detection of up to 5 targets in the same tube
Applications
CDTect Multiplex PCR Kits can be used for gene expression analysis of cDNA or quantification of gDNA on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Multiplex PCR Kit, which has been specially developed for fast cycling on these instruments.
