Product Name
CDFast Multiplex PCR Kits (2000)
Product Description
CDFast Multiplex PCR Kits enable fast and reliable quantification of up to 4 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote fast multiplex real-time PCR, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. Two kit formats are available: the CDFast Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDFast Multiplex PCR +R Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.
Components
For 2000 × 25 µL reactions: 25 mL 2 × CDFast Multiplex PCR Master Mix (with ROX dye), 20 mL RNase-Free Water
Advantage / Highlights
• For fast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
• Sensitive detection of up to 4 targets in 1 tube
• Faster results with time savings of up to 50%
• Successful multiplex PCR without the need for optimization
• Reliable quantification of low- and high-abundance targets
Applications
CDFast Multiplex PCR Kits can be used for multiplex gene expression analysis of cDNA or gDNA targets on any real-time cycler. This includes instruments from Agilent, Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, and Roche. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Multiplex PCR Kit, which has been specially developed for fast cycling on these instruments.
