Product Name
CDFast Probe PCR Kits
Product Description
CDFast Probe PCR Kits deliver fast and sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes. Q-bond technology and an optimized master mix enable shorter real-time PCR run times, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. Two kit formats are available: the CDFast Probe PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the CDFast Probe PCR +ROX Vial Kit for all other cyclers. For convenience, the master mix can be stored at 2-8 °C.
Components
For 4000 × 25 µL reactions: 2 × 25 mL 2 × CDFast Probe PCR Master Mix (contains ROX dye), 20 mL RNase-Free Water
Advantage / Highlights
• For fast, real-time PCR and two-step qRT-PCR using sequence-specific probes
• Sensitive detection of even low copy targets
• Accurate detection of a wide range of template amounts
• Faster results with a time savings of up to 60%
• Optimized, ready-to-use master mix for reliable results without optimization
• One cycling protocol for all standard and fast cyclers
Applications
CDFast Probe PCR Kits can be used for probe-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent. For the CD-Gene Q and other CD-Gene cyclers, we recommend using the CD-Gene Probe PCR Kit, which has been specially developed for fast cycling on these instruments.
