Product Name
CDNova Multiplex PCR Kits (100)
Product Description
CDNova Multiplex PCR Kits enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 hour. The combination of a unique hot start and PCR buffer system in the ready-to-use 4 × master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization, also providing automated reaction set up options at room temperature.
Components
For 100 × 20µL reactions: 500µL CDNova Multiplex PCR Mastermix, 500µL yellow template dilution buffer, 250µL ROX reference dye, 1.9µL RNase-Free water
Advantage / Highlights
• For ultrafast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
• Sensitive detection of up to 5 targets in 1 tube
• Reliable quantification of low- and high-abundance targets
• Up to 800ng template input for outstanding sensitivity
• Visual pipetting control preventing human errors
• Ultrafast and simple procedure increasing workflow efficiency
• Room temperature reaction set up allows automated procedures
Applications
CDNova Multiplex PCR Kits can be used for multiplex gene expression analysis of cDNA or gDNA targets on any real-time cycler. To fully exploit the capability of multiplex benefits, we recommend instruments providing up to 5-plex capacity, such as the CD-Gene Q.
