Product Name

HiScript II One Step qRT-PCR SYBR Green Kit

Cat No.

PCR-0362

Price

Inquiry

Product Description

The HiScript II One Step qRT-PCR SYBR Green Kit uses the SYBR Green I chimeric fluorescence method and is designed for quantitative PCR detection using RNA as a template (such as RNA viruses). Using gene-specific primers (GSP), reverse transcription and PCR reactions are performed in a single tube, eliminating the need for additional tube opening / pipetting operations, greatly increasing detection throughput and reducing the risk of contamination. Integrating the superior performance of HiScript II Reverse Transcriptase and Champagne Taq DNA Polymerase, combined with an optimized buffer system, the detection sensitivity can reach 1 pg total RNA. The patent specific enhancement factor added to the buffer can effectively reduce the formation of primer dimers and improve specificity.

Components

RNase free ddH₂O: 1.25 mL × 2 2 × One Step SYBR Green Mix: 1.25 mL × 2 One Step SYBR Green Enzyme Mix: 250 μL 50 × ROX Reference Dye 1: 100 μL 50 × ROX Reference Dye 2: 100 μL 2 × One Step SYBR Green Mix contains dNTP Mix, Mg ²⁺ specific enhancement factor, SYBR Green I. One Step SYBR Green Enzyme Mix contains HiScript Ⅱ Reverse Transcriptase, RNase inhibitor and Champagne Taq DNA Polymerase. The 50 × ROX Reference Dye is used to correct the fluorescence signal error between wells.

Advantage / Highlights

• Excellent specificity: special additive ingredients, to minimize the generation of primer dimers, more accurate quantitative results
• Ultra-high detection sensitivity: taking into account high amplification efficiency, can detect total RNA templates down to 1 pg

Applications

• This product is suitable for dye-based qRT-PCR using RNA as a template

Storage & Stability

Store at-20 ℃ in the dark