Product Name
CD gDNA Mini Tissue Kit
Product Description
The CD gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
Components
CD Lysis Buffer (L13)
CD Lysis Buffer (L15)
CD Magnetic Beads
Proteinase K (20 mg / mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg / mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
CD Purification Buffer (N5)
CD Wash Buffer (W12)
CD Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Advantage / Highlights
High Quality, High Yield Plasmid DNA
The CD gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg CDties of tissue typically in less than 15 minutes following sample preparation and lysis. CD gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 µg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.
Applications
The CD gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.
Storage & Stability
All components of the CD gDNA Tissue Kits are shipped at room temperature. Upon receipt, store the Proteinase K and RNase A at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.
Shipping conditions
Room Temperature
