Product Name
TaqMan miRNA ABC Purification Kit - Human Panel B
Product Description
Use the TaqMan miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.
The TaqMan miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B. The beads in each kit are superparamagnetic Dynabeads covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads.
Components
Lysis Buffer
ABC Buffer
Wash Buffer 1
Wash Buffer 2
Elution Buffer
1.5 mL LoBind tubes
Human Panel B Beads
Advantage / Highlights
• Simple and fast workflow using oligonucleotide-conjugated Dynal magnetic beads
• Small sample input: typical volumes range from 10 µL for blood samples to 50 µL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol reagent, or guanidine isothiocyanate
Applications
The miRNAs isolated using the TaqMan miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan microRNA assays or the TaqMan Array Human microRNA A and B cards.
Storage & Stability
Buffer Kit - Store at room temperature
Human Panel B Beads
Store at 2 °C to 8 °C
