TA cloning is a simple and convenient method of subcloning polymerase chain reaction (PCR) products. "TA" is short for "thymine" and "adenine."This cloning technique takes advantage of the terminal transferase activity of some DNA polymerases such as Taq polymerase, these enzymes preferentially add a 3'-end A-overhang to PCR products. This allows the direct insertion of such PCR products into the prelinearized cloning vector, which has a overhang on each 3'-end. This eliminates the need for restriction enzyme digestion of the vector or insert, primers with built-in restriction sites, or specially designed adapters, resulting in a much more efficient and robust cloning procedure.This technique is especially useful when compatible restriction sites are not available for the subcloning of DNA fragments.
Fig.1 TA Cloning
The research team of Creative Biogene has rich experience in the field of molecular biology and genetic engineering, which enables us to provide you with accurate and efficient services. At the same time, the high-performance PCR and RT-PCR technology of Creative Biogene enables us to clone longer and more complex DNA target segments. Creative Biogene can provide you with comprehensive TA cloning service, including template sequencing, PCR, expression vector construction, and protein expression and purification.
| Service items | Fragment length | Material requirements |
| Purified PCR products | 100 - 1500 bp | Average concentration: 15 ng / μ L, ≥ 15 μ L |
| 1500 - 3000 bp | Average concentration: 25 ng / μ L, ≥ 15 μ L | |
| Unpurified PCR products | 100 - 1500 bp | Average concentration: 30 ng / μ L, ≥ 30 μ L |
| 1500 - 3000 bp | Average concentration: 50 ng / μ L, ≥ 30 μ L |
Our TA cloning service offers the following delivery package:
You can customize the TA cloning service by phone or email, and our colleagues will reply your inquiry within three working days.