Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as rRNA, tRNA or snRNA genes, the product is a functional RNA. Gene expression analysis compares RNA expression levels of multiple genes at the same time. This analysis can help researchers identify the molecular basis of phenotypic differences and to select gene expression targets for in-depth study. It is a sensitive indicator of biological activity wherein a changing gene expression pattern is reflected in a change of biological process. As one of the most commonly used methods in research, clinical and pharmaceutical environment, it can lead to potential biomarker discovery for a particular disease phenotype and enable further biomarker validation.
Gene expression analysis can be achieved through a variety of means, however real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, and has little to no post-amplification processing.
As a supplier dedicated to the production and research of PCR products, the research team of Creative Biogene has professional basic knowledge in the field of real-time PCR and gene expression analysis. Creative Biogene can provide you with a full suite of gene expression analysis services including qPCR primer design and validation, qPCR reaction optimization, and complete data analysis. In addition, we can use SYBR green method or TaqMan probe method for gene expression analysis according to your requirements.
| Service items | Material requirements |
| Total RNA | ≥ 2 μg |
| Cell | ≥ 1× 106 μL |
| Flash frozen tissue | 2 - 5 g per sample |
| Plasmid DNA | ≥5 g |
You can customize the gene expression analysis service by phone or email, and our colleagues will reply your inquiry within three working days.