Messenger RNA is a kind of single strand RNA, which is transcribed from a strand of DNA as a template and carries genetic information and can guide protein synthesis. Based on the principle of complementary pairing of bases, mRNA is generated by transcription with the base in the cell as the template, which contains the base sequence corresponding to some functional segments of DNA molecules, and serves as a direct template for protein biosynthesis. mRNA accounts for 2% ~ 5% of total RNA in cells. RT-qPCR is a rapid, cheap and simple sensitive method for mRNA detection, it is considered the gold standard for accurate measurement of gene expression. Importantly, the ability to amplify multiple templates in a single reaction (multiple) makes it a true high-throughput detection method.
As a supplier dedicated to the production and research of PCR products, the research team of Creative Biogene has professional knowledge in the field of real-time PCR and mRNA expression analysis. Creative Biogene can provide you with complete mRNA expression analysis service by qPCR. The data analysis can also be carried out with our powerful bioinformatics platform.
| Service items | Material requirements |
| Tissue sample | ≥ 100 mg |
| Blood sample | volume ≥ 300 μL |
| Total RNA | ≥ 5 μg, OD value between 1.90 - 2.10 |
You can customize the mRNA expression analysis service by phone or email, and our colleagues will reply your inquiry within three working days.