SNP genotyping is a measure of the genetic variation of single nucleotide polymorphism (SNP) among species. SNP is one of the most common types of genetic variation. SNP is a single base pair mutation at a specific site, usually consisting of two alleles (rare allele frequency > 1%). SNP is associated with many human diseases including rheumatoid arthritis, diabetes, and multiple sclerosis. It is particularly important in pharmacogenetics. Real time PCR is an effective method for SNP genotyping. It provides a variety of markers for the detection of genetic variation, including DNA insertioners such as SYBR green, fluorescent probes and widely used TaqMan chemical methods.
Fig. 1 Schematic representation of TaqMan SNP genotyping. (Chris B, et al. 2010)
As a supplier dedicated to the production and research of PCR products, the research team of Creative Biogene has professional basic knowledge in the field of real-time PCR and SNP genotyping. Creative Biogene can provide you with professional SNP genotyping service using real-time PCR. The consequential analysis can also be carried out by our powerful bioinformatics platform.
| Service items | Material requirements |
| PCR products | Concentration ≥ 20ng / μL, volume ≥ 20 μL |
| Genomic DNA | concentration ≥ 20ng / μL, volume ≥ 20 μL |
| Blood sample | ≥ 500 μL anticoagulant; standard sampling tube is used, and EDTA is recommended for anticoagulant |
| Tissue sample | fresh (-80℃) or paraffin embedded tissue, fixed in 95% ethanol, with tissue amount ≥ 300mg |
You can customize the SNP genotyping service by phone or email, and our colleagues will reply your inquiry within three working days.